Prince Charles recently returned from a trip to the Middle East where he visited a refugee camp in Jordan which was home to hundreds of Syrians who have fled the fighting in their homeland.
The donation from the prince will be split between the Syrian Arab Red Crescent and the Jordanian Red Crescent.
The heir in line to the British throne has also made a donation to the DEC Syria appeal to boost aid for the refugees.
A Clarence House spokesperson said: “Earlier this month, the Prince of Wales and the Duchess of Cornwall learned first-hand about the plight of Syrian refugees when they visited the King Abdullah Park refugee camp in Jordan.
“During their visit they heard directly from some of the refugees about the terrible situation in their country, and they were especially moved when told of the traumatic experiences so many children had been through before and during their flight from the conflict in Syria.”